Molecular Formula: C10H12O4
Molecular Weight: 196.20
Appearance: Colorless glistening orthorhombic plates.
Thin Layer Chromatography: Silica Gel Plate Merck 60F254
: Eluent: CH2Cl2MeOH:H2O=86.5:10:0.75
: Detection: Iodine-saturated chamber: The spots are fixed with starch
: Result: 1 single spot with Rf ca. 0.76. The product is chromatographically pure.
Infrared Spectrum: Medium: KBr Tablet
: Concentration: 1/300
: Apparatus: Perkin-Elmer
: Result: The IR Spectrum exhibits the peak at WN>1800, which is typical of Anhydrides and it conforms with the data reported in literature for pure Cantharidin (Stork, G;van Tamelen,E. et al, J Am Chem Soc. 75.388 )
GLC: Solution 0.5% in Chloroform, 10 microlitre are injected.
: Column: 10% SILICA OV101 length: 1m
: Injector: 250°C
: Detector: 300°C
: Temperature: 180C for 20 minutes
: Result: 1 single peak, no side peaks. This product is GLC-pure
Melting Point: Found 216°C. Required by BPC (sealed tube, corrected) 216-218°C
Residue on Ignition: Negligible. Required by BPC less than 0.1%